标题： 明亮的亚20纳米阴极发光纳米探针用于多色电子显微镜 显示英文标题

标题： Bright sub-20 nm cathodoluminescent nanoprobes for multicolor electron microscopy

摘要： 电子显微镜（EM）在我们对从亚细胞结构到复杂生物体的生物系统的理解中起到了关键作用。 尽管EM能够以纳米级分辨率揭示细胞形态，但它无法提供蛋白质在细胞背景中的定位信息。 一种基于EM的生物成像技术，能够将单个蛋白质定位并解析蛋白质-蛋白质相互作用相对于细胞超结构的情况，将为细胞的分子生物学提供重要的见解。 在此，我们报告了发光纳米探针的开发，这些探针可能适用于多色EM模式下的生物分子标记。 在这种方法中，标记基于在电子激发下发光的稀土掺杂纳米颗粒，这一过程称为阴极发光（CL）。 我们的结果表明，通过优化纳米颗粒的组成、合成工艺和电子成像条件，可以实现亚20纳米分辨率的生物分子高信噪比定位，这仅受限于纳米颗粒的大小。 在集合测量中，这些发光标记表现出九种不同颜色的窄谱，这些颜色特征取决于相应的稀土掺杂类型。 显示英文摘要

摘要： Electron microscopy (EM) has been instrumental in our understanding of biological systems ranging from subcellular structures to complex organisms. Although EM reveals cellular morphology with nanoscale resolution, it does not provide information on the location of proteins within a cellular context. An EM-based bioimaging technology capable of localizing individual proteins and resolving protein-protein interactions with respect to cellular ultrastructure would provide important insights into the molecular biology of a cell. Here, we report on the development of luminescent nanoprobes potentially suitable for labeling biomolecules in a multicolor EM modality. In this approach, the labels are based on lanthanide-doped nanoparticles that emit light under electron excitation in a process known as cathodoluminescence (CL). Our results suggest that the optimization of nanoparticle composition, synthesis protocols and electron imaging conditions could enable high signal-to-noise localization of biomolecules with a sub-20-nm resolution, limited only by the nanoparticle size. In ensemble measurements, these luminescent labels exhibit narrow spectra of nine distinct colors that are characteristic of the corresponding rare-earth dopant type.