标题： 稳态福勒能量转移的Stern-Volmer模型在稀释自由扩散的膜结合荧光团之间的应用 显示英文标题

标题： Stern-Volmer Modeling of Steady-State Forster Energy Transfer Between Dilute, Freely Diffusing Membrane-Bound Fluorophores

摘要： 用于评估荧光团在稳态下的福斯特共振能量转移（FRET）的两种不同度量是：(1) 受体对供体荧光的淬灭，E（也称为转移效率）；以及 (ii) 供体激发的受体荧光，F-A-Dex。 虽然E仍然更广泛使用，但F-A-Dex由于实际原因在研究生物膜的实验者中越来越受欢迎。 在这里，对于膜结合荧光团的特殊情况，我们提供了大量的实验证据，证明在稀释探针条件下，当对任一FRET度量进行建模时，使用简单的斯托克斯-沃尔默表达式是合理的。 我们还发现，我们的E的斯托克斯-沃尔默表达式与Wolber和Hudson对二维稳态福斯特淬灭的级数近似之间存在稀释区间的对应关系。 这种新颖的对应关系使我们能够根据（i）一个有效的福斯特距离，以及（ii）两个最大受体浓度极限来解释我们的二维淬灭常数，每个极限定义了其自身的有用实验区间。 综合来看，我们的结果表明了一种三步策略，以设计更有效的稳态FRET实验来研究生物膜。 显示英文摘要

摘要： Two different metrics are used to assess Forster resonance energy transfer (FRET) between fluorophores in the steady state: (1) acceptor-quenching of donor fluorescence, E (a.k.a. transfer efficiency); and (ii) donor-excited acceptor fluorescence, F-A-Dex. While E is still more widely used, F-A-Dex has been gaining in popularity for practical reasons among experimentalists who study biomembranes. Here, for the special case of membrane-bound fluorophores, we present a substantial body of experimental evidence that justifies the use of simple Stern-Volmer expressions when modeling either FRET metric under dilute-probe conditions. We have also discovered a dilute-regime correspondence between our Stern-Volmer expression for E and Wolber and Hudson's series approximation for steady-state Forster quenching in 2D. This novel correspondence allows us to interpret each of our 2D quenching constants in terms of both (i) an effective Forster distance, and (ii) two maximum acceptor-concentration limits, each of which defines its own useful experimental regime. Taken together, our results suggest a three-step strategy toward designing more effective steady-state FRET experiments for the study of biomembranes.