标题： 用于将羟基磷灰石固定在3D打印多孔聚醚醚酮植入物上的单步方法 显示英文标题

标题： Single-step method for the immobilization of hydroxyapatite on 3D-printed porous polyetherketoneketone implants

摘要： 组织工程结构（支架）的发展用于骨组织缺损的重建是现代生物材料科学的相关任务。 与基于金属的结构相比，聚合物结构提供了许多优点，其中包括更好的可加工性和避免金属沉着。 由于其优异的机械性能和生物相容性，聚醚醚酮（PEKK）成为开发此类结构的一种有前景的材料。 此前，我们小组提出了一种将羟基磷灰石（HAp）固定在PEKK表面的方法，以增强干细胞的粘附。 在本研究中，我们提出了一种单步方法，将HAp固定在3D打印多孔PEKK植入物的表面。 所提出的方法能够保持原始植入物的形态（孔径、打印线条的宽度）。 此外，样品表面最多有35.0±14.0%被HAp颗粒覆盖，这导致了改善的亲水性（0度水接触角）。 改性样品表面的钙和磷含量分别高达17.4±4.1和8.0±1.7 wt.%。 重要的是，所提出的改性方法保持了3D打印多孔PEKK植入物的抗压强度。 HAp的固定提高了干细胞的粘附性（从121±40个/平方毫米增加到234±8个/平方毫米），并诱导了它们的成骨分化。 显示英文摘要

摘要： The development of tissue engineering structures (scaffolds) for the reconstruction of bone tissue defects is the relevant task of modern biomedical materials science. Compared to metal-based structures, polymer constructs provide numerous advantages, among them - better processibility and metallosis avoidance. Owing to its high mechanical performance and biocompatibility, polyetherketoneketone (PEKK) became a promising material for the development of such structures. Previously, a method for the immobilization of hydroxyapatite (HAp) on PEKK surface was proposed by our group for the enhancement of stem cell adhesion. In the present study, we propose a single-step method of HAp immobilization on the surface of 3D-printed porous PEKK implants. The proposed approach allowed to preserve the morphology (pore diameter, width of the printed lines) of the pristine implants. With that, up to 35.0+-14.0 % of the sample surface were coated with HAp particles, which resulted in improved hydrophilicity (0 degrees water contact angle). The calcium and phosphorus content on the surface of the modified samples was up to 17.4+-4.1 and 8.0+-1.7 wt. %, respectively. Importantly, the proposed modification preserved compressive strength of the 3D-printed porous PEKK implants. HAp immobilization provided better adhesion of stem cells (from 121+-40 cells/mm2 to 234+-8 cells/mm2) and induce their osteogenic differentiation.