标题： 数字成像质谱 显示英文标题

标题： Digital Imaging Mass Spectrometry

摘要： 通过质谱成像技术可视化分子的二维分布的方法发展迅速，并在生物学、医学和材料表面科学中产生了新的应用。 大多数质谱成像设备逐点获取高质谱分辨率的光谱，从而扫描物体表面。 因此，成像速度较慢，图像重建仍然繁琐。 在这里，我们描述了一种成像质谱仪，它通过离子光学手段利用飞行时间质谱分离的真实成像能力。 该质谱仪配备了ASIC Timepix芯片作为阵列探测器，以直接从靶样品上成像的离子获取位置、质量和强度。 这种成像质谱仪在样品上的空间分辨率为(84\pm 35) \mu m，质谱分辨率为45，并能够在高达约2 cm²的表面上定位原子或有机化合物。 在结构化样品上约5 mm²的扩展激光光斑允许选定质量的并行成像。 数字成像质谱仪证明了高命中多重性、简单的图像重建以及以4 kHz或更高的速度读取的潜力。 该装置展示了一种类似于数字照相机的真实图像采集的简单方法。 这项技术可能使未来对生物分子样品的快速分析成为可能。 显示英文摘要

摘要： Methods to visualize the two-dimensional distribution of molecules by mass spectrometric imaging evolve rapidly and yield novel applications in biology, medicine, and material surface sciences. Most mass spectrometric imagers acquire high mass resolution spectra spot-by-spot and thereby scan the object's surface. Thus, imaging is slow and image reconstruction remains cumbersome. Here we describe an imaging mass spectrometer that exploits the true imaging capabilities by ion optical means for the time of flight mass separation. The mass spectrometer is equipped with the ASIC Timepix chip as an array detector to acquire the position, mass, and intensity of ions that are imaged by MALDI directly from the target sample onto the detector. This imaging mass spectrometer has a spatial resolving power at the specimen of (84\pm35) \mu m with a mass resolution of 45 and locates atoms or organic compounds on a surface area up to ~2 cm2. Extended laser spots of ~5 mm2 on structured specimens allowed parallel imaging of selected masses. The digital imaging mass spectrometer proves high hit-multiplicity, straightforward image reconstruction and its potential for high-speed readout at 4 kHz or more. This device demonstrates a simple way of true image acquisition like a digital photographic camera. The technology may enable a fast analysis of biomolecular samples in near future.