标题： 通过2,4-二硝基氟苯柱前衍生化分析发芽糙米中的γ-氨基丁酸 显示英文标题

标题： Analysis of gamma-amino butyric acid in the germinated brown rice by pre-column derivatization with 2, 4-dinitrofluorobenzene

摘要： 本文报告了通过液相色谱法分析发芽糙米中的γ-氨基丁酸的方法。 GABA通过与2,4-二硝基氟苯进行柱前衍生化处理。 为了分离含有各种氨基酸的发芽糙米提取物中的GABA，我们回顾了分离柱种类和超高效液相色谱流动相梯度的影响。 GABA从发芽糙米中用蒸馏水提取，最佳提取时间为12小时。为验证该方法，讨论了精密度和回收率。 在5至50微克每毫升的浓度范围内，GABA的校准曲线呈线性，回归方程的相关系数为0.999。 通过氨基酸分析仪对GABA进行分析，并与该方法进行比较，F检验和t检验的结果表明两种方法在准确度和精密度上没有显著差异。 显示英文摘要

摘要： This paper reports the analytical method for gamma-amino butyric acid in the germinated brown rice by liquid chromatography. GABA was derivatized by pre-column derivatization with 2, 4-dinitrofluorobenzene. To separate GABA in the germinated brown rice extracts containing various amino acids, we reviewed the influence of the kind of separation columns and the gradient of mobile phase of ultra performance liquid chromatography. GABA was extracted with distilled water from the germinated brown rice with the optimum extraction time of 12 h. To validate this method the precision and the recovery were discussed. In the concentration range from 5 to 50 microgram per mililiter, the calibration curve for GABA was linear and the regression equation was obtained with correlation coefficient of 0.999. GABA was analyzed by amino acid analyzer in comparison with this method and the results of F-test and t-test indicated that there were no significant differences in accuracy and precision between two methods.