标题： 冷等离子体释放拟南芥种子休眠依赖于细胞质玻璃化转变 显示英文标题

标题： Release of Arabidopsis seed dormancy by cold atmospheric plasma relies on cytoplasmic glass transition

摘要： 当成熟的拟南芥种子处于休眠状态时，在看似有利的条件下其发芽会被阻止。 这种初始休眠可以通过一种称为后熟的过程在种子干燥储存期间被解除，该过程可能持续数月。 为了减少这一延迟，可以使用冷大气等离子体（CAP）作为活性氧物种的来源，能够引发异质化学反应。 虽然已知CAP能够刺激各种种子种类的发芽，但CAP处理与干种子非晶态固体状态之间的关系仍未被探索。 在这里，我们证明可以使用环境空气的冷等离子体缓解种子休眠，并且对于含水量高（通常为30% DW）的种子或在等离子体处理过程中加热至60 {\deg }C的种子，这种缓解效果可以被放大。 差示扫描微热量计显示，这些特性控制着种子细胞质的玻璃态/橡胶态。 该技术还表明，向橡胶态的玻璃转变增强了CAP缓解种子休眠的效果。 我们认为，较低的细胞质粘度可以促进由CAP引起的氧化信号传导，从而改善发芽过程。 显示英文摘要

摘要： When mature Arabidopsis thaliana seeds are dormant, their germination is prevented in apparently favourable conditions. This primary dormancy can be released during seed dry storage through a process called after-ripening whose duration can last several months. To reduce this delay, cold atmospheric plasmas (CAP) can be used as sources of reactive oxygen species capable of inducing heterogeneous chemical reactions. While CAP are known to stimulate the germination of various seed species, the relationship between CAP treatments and the amorphous solid state of dry seeds remains unexplored. Here, we demonstrate that seed dormancy can be alleviated using a cold plasma of ambient air and that this alleviation can be amplified for seeds with high water-content (typically 30 %DW) or seeds heated at 60 {\deg}C during plasma treatment. Differential scanning micro-calorimetry shows that these characteristics control the glassy/rubbery state of the seed cytoplasm. This technique indicates also that a glass transition to the rubbery state strengthens the CAP effects to alleviate seed dormancy. We propose that lower cytoplasmic viscosity can promote the oxidative signaling induced by CAP which, in turn, improves the germination process.