标题： 多谱系分化抗应激细胞通过分泌TGF-β和IL-10缓解小鼠神经病理性疼痛 显示英文标题

标题： Multilineage-differentiating stress-enduring cells alleviate neuropathic pain in mice by secreting TGF-b and IL-10

摘要： 神经病理性疼痛是一种慢性疾病，其特征是周围或中枢神经系统受损和功能障碍。 目前尚无有效的治疗选择用于神经病理性疼痛，现有药物通常仅能提供暂时的缓解，并可能产生副作用。 多谱系分化耐压（Muse）细胞具有高扩增潜力、稳定的表型和强效的免疫抑制作用。 这些特性使它们成为治疗神经病理性疼痛管理的有吸引力的候选药物。 在本研究中，我们进行了一系列实验来评估Muse细胞对神经病理性疼痛的影响。 来自不同物种的Muse细胞通过逆转CCI诱导的神经病理性疼痛表现出镇痛潜力。 蛋白质分析显示Muse细胞与BMSCs之间存在高度相似性。 Muse细胞的鞘内注射在各种小鼠模型中有效减轻了神经病理性疼痛，产生的镇痛效果优于等量低剂量BMSCs的给药。 免疫组织化学分析和qPCR揭示了Muse细胞抑制SNI引起的脊髓神经炎症的能力。 此外，Transwell和ELISA显示Muse细胞通过CCR7-CCL21趋化轴迁移到受损的背根神经节（DRG）。 此外，Muse细胞分泌TGF-b和IL-10被确定为Muse细胞镇痛作用的机制。 Muse细胞通过调节TGF-b和IL-10减轻神经炎症并产生镇痛效果的能力，突显了它们作为治疗神经病理性疼痛的有希望的治疗方法的潜力。 显示英文摘要

摘要： Neuropathic pain is a chronic condition characterized by damage to and dysfunction of the peripheral or central nervous system. There are currently no effective treatment options available for neuropathic pain, and existing drugs often provide only temporary relief with potential side effects. Multilineage-differentiating stress-enduring (Muse) cells are characterized by high expansion potential, a stable phenotype and strong immunosuppression. These properties make them attractive candidates for therapeutics for neuropathic pain management. In this study, we conducted a series of experiments to evaluate the effect of Muse cells on neuropathic pain. Muse cells from different species demonstrated analgesic potential by reversing CCI-induced neuropathic pain. Protein profiling revealed a high degree of similarity between Muse cells and BMSCs. The intrathecal injection of Muse cells effectively reduced neuropathic pain in various mouse models, resulting in better analgesic effects than the administration of equivalent low doses of BMSCs. Immunohistochemical analysis and qPCR revealed the ability of Muse cells to inhibit spinal cord neuroinflammation caused by SNI. In addition, Transwell and ELISA revealed that Muse cells migrated through the injured dorsal root ganglion (DRG) via the CCR7-CCL21 chemotactic axis. In addition, the secretion of TGF-b and IL-10 by Muse cells was identified as the mechanism underlying the analgesic effect of Muse cells. The capacity of Muse cells to mitigate neuroinflammation and produce analgesic effects via the modulation of TGF-b and IL-10 underscores their potential as promising therapeutic approaches for the treatment of neuropathic pain.