标题： 高温1H DOSY NMR显示，小麦粉的酸面团发酵改变了水溶性阿拉伯木聚糖的分子结构 显示英文标题

标题： High temperature 1H DOSY NMR reveals sourdough fermentation of wheat flour alters the molecular structure of water-extractable arabinoxylans

摘要： 阿拉伯木聚糖是小麦粉的成分，对小麦的膳食纤维特性有贡献。 它们存在水溶性和水不溶性形式，并对人类健康有贡献。 在烘焙技术中，特别是水溶性阿拉伯木聚糖（WE-AX）由于其对粘度和面团流变学的影响而很重要。 本研究提供了关于小麦粉发酵对酸面团生产过程中WE-AX影响的见解，为改善酸面团面包质量和健康益处提供了潜在应用。 众所周知，酸面团的生产会增加WE-AX部分，但其背后的（生物）化学机制仍不清楚。 本研究利用1H扩散有序光谱（DOSY）核磁共振（NMR）在高温下分析了不同乳酸菌（LAB）菌株在酸面团生产过程中小麦粉发酵期间WE-AX的结构变化。 结果证实，在高温下的DOSY NMR大大提高了该方法在分析较大生物分子方面的适用性。 总体而言，发现随着发酵时间的增加，WE-AX化合物的尺寸减小。 这通过较高自扩散系数的出现以及横向弛豫时间的增加得到了说明。 需要进一步的研究来解释与一般趋势的偏差。 显示英文摘要

摘要： Arabinoxylans are constituents of wheat flour that contribute to the dietary fiber properties of wheat. They exist in water-extractable and water-unextractable forms and contribute to human health. In bakery technology, especially the water-extractable arabinoxylans (WE-AX) are important due to their impact on viscosity and dough rheology. This study provides insights into the impact of wheat flour fermentation on WE-AX during sourdough production, offering potential applications for improving sourdough bread quality and its health benefits. The production of sourdoughs is known to increase the WE-AX fraction, yet the underlying (bio)chemical mechanisms remain unclear. This study investigated the alteration of WE-AX during the fermentation of wheat flour for sourdough production using 1H Diffusion Ordered SpectroscopY (DOSY) Nuclear Magnetic Resonance (NMR) at elevated temperature to analyze the structural changes of WE-AX during wheat flour fermentation for sourdough production with different lactic acid bacteria (LAB) strains. The results confirmed that DOSY NMR at elevated temperatures greatly improved the applicability of the method for analyzing larger biomolecules. Overall, a size reduction of the WE-AX compounds with increasing fermentation time was found. This was indicated both by the occurrence of higher self-diffusion coefficients, and increased transverse relaxation times. Further research is necessary to explain deviations from the general trend.