Title: Intermittent flow paths in biofilms grown in a microfluidic channel Show Chinese title

Title: 微流控通道中生长的生物膜中的间歇性流道

Abstract: Biofilms exposed to flow experience shear stress, which leads to a competitive interaction between the growth and development of a biofilm and shearing. In this study, Pseudonomas fluorescene biofilm was grown in a microfluidic channel and exposed to forced flow of an aqueous solution of variable velocity. It can be observed that under certain conditions preferential flow paths form with a dynamic, but quasi-steady state interaction of growth, detachment, and re-attachment. We find that the regimes for preferential flow path development are determined by nutrient availability and the ratio of shear stress versus the biofilm's ability to resist shear forces. The intermittent regime of flow paths is mainly driven by the supply with nutrients, which we confirm by comparison with a numerical model based on coarse-grained molecular dynamics and Lattice Boltzmann hydrodynamics. Show Chinese abstract

Abstract: 暴露于流动中的生物膜会经历剪切应力，这导致生物膜的生长与发育与其受到剪切作用之间存在竞争关系。 在这项研究中，铜绿假单胞菌生物膜在微流控通道中生长，并暴露于具有可变流速的水溶液强制流动下。 可以观察到，在某些条件下，形成了优先流动路径，并且生长、脱落和重新附着之间存在动态但准稳态的相互作用。 我们发现，优先流动路径的发展区域由营养物质的可用性以及剪切应力与生物膜抵抗剪切力的能力之比决定。 间歇性的流动路径主要受营养供应驱动，这一点通过与基于粗粒分子动力学和格子玻尔兹曼流体力学的数值模型对比得到了证实。