标题： 溶液中单个纳米尺度胞外囊泡和纳米颗粒的快速捕获与无标记表征 显示英文标题

标题： Rapid Trapping and Label-free Characterization of Single Nanoscale Extracellular Vesicles and Nanoparticles in Solution

摘要： 实现对单个纳米颗粒的高通量、全面分析以确定其大小、形状和成分，对于理解粒子异质性至关重要，其应用范围从药物递送到环境监测。 现有的技术受到低通量、长时间捕获、不可逆的颗粒吸附或有限的表征能力的阻碍。 在这里，我们介绍了干涉电动力镊子（IET），这是一种集成平台，能够在三秒内并行快速捕获单个纳米颗粒。 IET 通过干涉成像实现无标记的颗粒大小和形状表征，并通过拉曼光谱识别分子组成，无需荧光标记。 我们通过捕获和成像胶体聚合物珠、纳米尺度的细胞外囊泡（EVs）以及新发现的被称为超级体的细胞外纳米颗粒来展示该平台的能力。 通过监控它们被捕获时的干涉对比度图像，我们准确地确定了 EVs 和超级体的大小。 我们的 IET 是一种强大的光电流体平台，用于全面表征纳米级对象，开辟了纳米医学、环境监测等领域的新途径。 显示英文摘要

摘要： Achieving high-throughput, comprehensive analysis of single nanoparticles to determine their size, shape, and composition is essential for understanding particle heterogeneity with applications ranging from drug delivery to environmental monitoring. Existing techniques are hindered by low throughput, lengthy trapping times, irreversible particle adsorption, or limited characterization capabilities. Here, we introduce Interferometric Electrohydrodynamic Tweezers (IET), an integrated platform that rapidly traps single nanoparticles in parallel within three seconds. IET enables label-free characterization of particle size and shape via interferometric imaging and identifies molecular composition through Raman spectroscopy, all without the need for fluorescent labeling. We demonstrate the platform's capabilities by trapping and imaging colloidal polymer beads, nanoscale extracellular vesicles (EVs), and newly discovered extracellular nanoparticles known as supermeres. By monitoring their interferometric contrast images while trapped, we accurately determine the sizes of EVs and supermeres. Our IET represents a powerful optofluidics platform for comprehensive characterization of nanoscale objects, opening new avenues in nanomedicine, environmental monitoring, and beyond.