标题： 单分子荧光爆发在微/纳米流体通道中的最佳漂移速度 显示英文标题

标题： Optimum Drift Velocity for Single Molecule Fluorescence Bursts in Micro/Nano-Fluidic Channels

摘要： 光子脉冲直方图可用于在微/纳米流体通道中识别单个蛋白质分子，前提是直方图的宽度较窄。 由纵向扩散引起的光子散粒噪声和驻留时间波动是直方图宽度的主要来源。 本通讯是我们在之前的一封信件[L. L. Kish 等，Appl. Phys. Lett. 99, 143121 (2011)]的后续，并展示了对于给定的扩散系数，漂移速度的增加会增强相对散粒噪声并减少相对驻留时间波动。 这导致了一个最优的漂移速度，该速度可最小化直方图宽度并最大化识别单个分子的能力，这对于应用来说是一个重要的结果。 显示英文摘要

摘要： Photonic burst histograms can be used to identify single protein molecules in micro/nano-fluidic channels provided the width of the histogram is narrow. Photonic shot noise and residence time fluctuations, caused by longitudinal diffusion, are the major sources of the histogram width. This Communication is a sequel to an earlier Letter of ours [L. L. Kish et al., Appl. Phys. Lett. 99, 143121 (2011)] and demonstrates that, for a given diffusion coefficient, an increase of the drift velocity enhances the relative shot noise and decreases the relative residence time fluctuations. This leads to an optimum drift velocity which minimizes the histogram width and maximizes the ability to identify single molecules, which is an important result for applications.