标题： 与多柔比星偶联的超顺磁性氧化铁纳米颗粒用于靶向乳腺癌 显示英文标题

标题： Superparamagnetic iron oxide nanoparticles conjugated with doxorubicin for targeting breast cancer

摘要： 下一代癌症治疗模式的发展目前是肿瘤学的一个关键需求。 纳米技术的进步使我们更接近于开发出具有双重和多功能特性的纳米颗粒，这些纳米颗粒正在挑战传统诊断和治疗剂之间的区分。 追求创新、多功能、更高效和更安全的治疗方案是乳腺癌前临床纳米颗粒介导的热疗研究中的主要挑战。 在此，我们报告铁氧化物纳米颗粒具有作为磁性和药物输送剂的双重能力。 本研究的目的是研究载有阿霉素（DOX）的带负电荷的聚羧酸铁氧化物纳米颗粒（SPIONs）以及罗丹明B功能化的SPIONs对乳腺癌细胞系的体外影响。 为了正确分析和理解与游离DOX相比，DOX-SPIONs给药后细胞行为的变化，使用了一套复杂的体外测试，包括MTT测定、细胞周期测定和细胞摄取。 总之，我们开发了一种基于磁性纳米颗粒的药物输送系统，该系统能够将细胞毒性药物阿霉素顺序递送至乳腺癌细胞（MCF-7和MDA-MB-231）。 药物涂层纳米颗粒DOX-NPs是逐步组装的，首先通过静电反应将阿霉素吸附到裸铁氧化物纳米颗粒上，从而允许阿霉素的复合。 相反，它们在MCF-7和MDA-MB-231细胞中被内化得多得多，并由于两种药物的协同作用和高温效应而具有细胞毒性。 药物涂层颗粒能够在体外抑制乳腺癌细胞的生长和增殖，表明该系统有潜力作为一种抗转移的化疗热疗剂。 显示英文摘要

摘要： Development of the next generations of cancer therapy modalities is currently a crucial requirement of oncology. Advances in nanotechnology are bringing us closer to the development of dual and multifunctional nanoparticles that are challenging the traditional distinction between diagnostic and treatment agents. The chase of innovative, multifunctional, more efficient, and safer treatments is a major challenge in preclinical nanoparticle-mediated thermotherapeutic research for breast cancer. Here, we report that iron oxide nanoparticles have the dual capacity to act as both magnetic and drug delivery agents. The aim of this work was to investigate the in vitro effect of the loading of doxorubicin (DOX) on negatively charged polycarboxylic iron-oxide nanoparticles (SPIONs) and Rhodamine B functionalized SPIONs on breast carcinoma cell lines. For proper analysis and understanding of cell behavior after administration of DOX-SPIONs compared with free DOX, a complex set of in vitro tests, including production of MTT assay, cell cycle determination, and cellular uptake, were utilized. In summary, we have developed a magnetic nanoparticle-based drug delivery system that sequentially delivers the cytotoxic drug doxorubicin to breast cancer cells (MCF-7 and MDA-MB-231). The drug-coated nanoparticles, DOX-NPs, were assembled stepwise, with doxorubicin adsorbed to bare iron oxide nanoparticles first, by electrostatic reaction and allowed for the complexation of doxorubicin. In contrast, they were internalized to a much greater extent in MCF-7 and MDA-MB-231 cells and were cytotoxic due to the synergistic action of the two drugs and the effects of hyperthermia. The drug-coated particles were able to inhibit growth and proliferation of breast cancer cells in vitro, indicating that the system has potential to act as an antimetastatic chemothermotherapeutic agent.