标题： pgMAP：一种用于从双靶向CRISPR筛选中实现引导RNA读段映射的流程 显示英文标题

标题： pgMAP: a pipeline to enable guide RNA read mapping from dual-targeting CRISPR screens

摘要： 我们开发了pgMAP，一个分析流程，用于映射双靶点CRISPR筛选中的gRNA测序读数。 pgMAP输出包括一个双gRNA读数计数表和质量控制指标，包括正确配对读数的比例以及所有时间点和样本中CRISPR文库的测序覆盖度。 pgMAP使用Snakemake实现，并在https://github.com/fredhutch/pgmap_pipeline下以MIT许可证开源可用。 显示英文摘要

摘要： We developed pgMAP, an analysis pipeline to map gRNA sequencing reads from dual-targeting CRISPR screens. pgMAP output includes a dual gRNA read counts table and quality control metrics including the proportion of correctly-paired reads and CRISPR library sequencing coverage across all time points and samples. pgMAP is implemented using Snakemake and is available open-source under the MIT license at https://github.com/fredhutch/pgmap_pipeline.