标题： 使用布里渊显微镜区分野生型和CRISPR修饰的结肠癌细胞 显示英文标题

标题： Differentiation of Wild-Type and CRISPR-Modified Colon Cancer Cells Using Brillouin Microscopy

摘要： 本研究通过布里渊显微镜研究结肠癌细胞的力学特性，重点在于野生型（WT）和CRISPR修饰细胞之间的区分。布里渊微光谱技术是一种非侵入性技术，用于测量体外细胞的布里渊位移和半高全宽（FWHM）值。使用自建的共聚焦布里渊微光谱仪，野生型和CRISPR修饰细胞表现出不同的力学响应。统计分析显示，野生型细胞的刚度和粘度与CRISPR修饰细胞不同，这由它们的布里渊位移和FWHM值表明。数据表明，布里渊光谱提供了一种可行的方法，在亚细胞水平上区分正常细胞和突变细胞，为癌症研究相关的细胞力学特性提供了新的见解。这些发现有望推动非侵入性诊断技术的发展，并加深对肿瘤学中细胞力学的理解。 显示英文摘要

摘要： This study investigates the mechanical properties of colon cancer cells through Brillouin microscopy, focusing on the differentiation between wild-type (WT) and CRISPR-modified cells. Brillouin microspectroscopy, a non-invasive technique, was employed to measure Brillouin shifts and full width at half maximum (FWHM) values of the cells in vitro. Using a custom-built confocal Brillouin microspectrometer, both WT and CRISPR-modified cells exhibited distinct mechanical responses. Statistical analysis revealed that WT cells had different stiffness and viscosity compared to CRISPR-modified cells, as indicated by their Brillouin shift and FWHM values. The data suggest that Brillouin spectroscopy offers a viable method to differentiate between normal and mutated cells at the subcellular level, providing new insights into cellular mechanical properties relevant to cancer research. These findings hold potential for advancing non-invasive diagnostic techniques and understanding cellular mechanics in oncology.