标题： 等离子体彩色滤光器实现亚衍射极限分辨率的无标记等离子体增强阵列断层扫描 显示英文标题

标题： Plasmonic Color Filters Enable Label-Free Plasmon-Enhanced Array Tomography with sub-diffraction limited resolution

摘要： 三维（3D）成像对于理解细胞和组织的亚细胞结构和形态至关重要。 尽管染色是最广泛用于在显微镜下观察生物样本的方法，但细胞内折射率（RI）已被提出作为一种潜在的生物物理标记，可能补充甚至超越当前组织学方法的灵敏度。 因此，开发新的、高度敏感的无标签技术，能够检测细胞内折射率的变化，在生物医学成像中是非常迫切的。 等离子体超材料的近期发展，旨在模仿传统的载玻片，使得将折射率的细微变化直接转化为颜色成为可能。 这种方法使使用标准组织学载玻片制备方法实现了无标签的组织微结构可视化。 在这里，我们展示了超薄切片机辅助的光学等离子体增强（PE）阵列断层扫描，并将其与相同样品在树脂中的电子阵列断层扫描相关联。 该方法实现了30-200 nm范围内的轴向超分辨无标签全细胞成像，并显示出在（亚）细胞器水平上多模式三维比色组织学的巨大潜力。 显示英文摘要

摘要： Three-dimensional (3D) imaging of the subcellular organisation and morphology of cells and tissues is essential for understanding biological function. Although staining is the most widely used approach for visualising biological samples under a microscope, the intracellular refractive index (RI) has been proposed as a potential biophysical marker that could supplement or even surpass the sensitivity of current histological methods. Hence, the development of new, highly sensitive, label-free techniques that can detect changes in the intracellular RI is extremely desirable for biomedical imaging. The recent development of plasmonic metamaterials, designed to mimic a traditional microscope slide, have made it possible to translate subtle changes in refractive index directly into color. This approach enables label-free visualization of tissue microstructure using standard histological slide preparation methods. Here we demonstrate ultramicrotome-assisted optical plasmon-enhanced (PE) array tomography and correlate this with electron array tomography of the same sample embedded in resin. The approach enables axially super-resolved label-free imaging of whole cells in the range of 30-200 nm and shows great potential for multimodal three-dimensional colorimetric histology at the (sub-) organelle level.