标题： 光片显微镜评估癌症病理学：当前观点和未来趋势 显示英文标题

标题： Light-sheet microscopy to assess cancer pathology: current views and future trends

摘要： 近年来，光片荧光显微镜（LSFM）已成为一种强大的工具，用于可视化和分析癌症组织样本，包括患者来源的标本、类器官、活检和小鼠模型。 在本工作中，我们强调了深度组织LSFM在肿瘤学中的当前应用，并展示了其在多种人类癌症组织中的使用，包括但不限于前列腺和乳腺。 在此，我们讨论了将先进的LSFM技术整合到临床工作流程中的潜力，以实现完整癌症标本的高通量三维成像。 这种方法具有显著的潜力，可以提高诊断精度，并提供关于肿瘤结构和形态的新见解。 显示英文摘要

摘要： In recent years, light-sheet fluorescence microscopy (LSFM) has emerged as a powerful tool for visualizing and analyzing cancer tissue samples, including patient-derived specimens, organoids, biopsies, and murine models. In this work, we highlight the current applications of deep tissue LSFM in oncology and illustrate its use across a variety of human cancer tissues, including - but not limited to - prostate and breast. Here, we discuss that the potential integration of advanced LSFM technologies into clinical workflows to enable high-throughput, three-dimensional imaging of intact cancer specimens. This approach holds significant potential to enhance diagnostic precision and provide novel insights into tumour architecture and morphology.