标题： 膜蛋白和蛋白质组学：爱是可能的，但也很困难 显示英文标题

标题： Membrane proteins and proteomics: Love is possible, but so difficult

摘要： 尽管经过数十年的广泛研究，膜蛋白的大规模分析仍然是一个困难的任务。 这是由于膜蛋白需要一个精心平衡的亲水和疏水环境，而这种最佳环境因不同的蛋白质而异，而大多数蛋白质化学方法主要（如果不是唯一的话）在水基介质中进行。 以这篇综述[Santoni, Molloy 和 Rabilloud, 膜蛋白与蛋白质组学：一种不可能的爱情？Electrophoresis 2000, 21, 1054-1070]作为关键论文，本文分析了膜蛋白质组学领域如何加剧了蛋白质组学中的趋势，即开发替代传统二维电泳的方法，从而越来越多地给质谱学方面带来压力。 然而，在膜蛋白的情况下，这样做的动机是由于膜蛋白的溶解性较差。 这篇综述还表明，在某些情况下，当溶解性问题不那么严重时，二维电泳仍然是首选方法。 最后但同样重要的是，这篇综述还对用于膜蛋白蛋白质组学分析的替代方法进行了批判性审查。 显示英文摘要

摘要： Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with different proteins, while most protein chemistry methods work mainly, if not only, in water-based media. Taking this review [Santoni, Molloy and Rabilloud, Membrane proteins and proteomics: un amour impossible? Electrophoresis 2000, 21, 1054-1070] as a pivotal paper, the current paper analyzes how the field of membrane proteomics exacerbated the trend in proteomics, i.e. developing alternate methods to the historical two-dimensional electrophoresis, and thus putting more and more pressure on the mass spectrometry side. However, in the case of membrane proteins, the incentive in doing so is due to the poor solubility of membrane proteins. This review also shows that in some situations, where this solubility problem is less acute, two-dimensional electrophoresis remains a method of choice. Last but not least, this review also critically examines the alternate approaches that have been used for the proteomic analysis of membrane proteins.