标题： 基于电极固定葡萄糖-6-磷酸脱氢酶的酶促与门：面向低噪声数字生物传感器和生化逻辑系统的研究 显示英文标题

标题： Enzymatic AND-Gate Based on Electrode-Immobilized Glucose-6-Phosphate Dehydrogenase: Towards Digital Biosensors and Biochemical Logic Systems with Low Noise

摘要： 固定在电极上的葡萄糖-6-磷酸脱氢酶用于催化进行与逻辑门的酶促反应。 此逻辑功能在此背景下考虑，用于生物催化过程，以实现生物计算应用中的“数字”（阈值）传感/执行。 我们概述了此类应用所需的响应函数，并报告了其中一个输入中首次实验实现的S形响应。 开发并利用了一个动力学模型，以评估实验实现的门电路接近最优的程度。 显示英文摘要

摘要： Electrode-immobilized glucose-6-phosphate dehydrogenase is used to catalyze an enzymatic reaction which carries out the AND logic gate. This logic function is considered here in the context of biocatalytic processes utilized for the biocomputing applications for "digital" (threshold) sensing/actuation. We outline the response functions desirable for such applications and report the first experimental realization of a sigmoid-shape response in one of the inputs. A kinetic model is developed and utilized to evaluate the extent to which the experimentally realized gate is close to optimal.