标题： 纳米金刚石作为将siRNA递送至尤文氏肉瘤细胞的载体 显示英文标题

标题： Nanodiamond as a vector for siRNA delivery to Ewing sarcoma cells

摘要： 我们研究了金刚石纳米颗粒（纳米金刚石，NDs）在Ewing肉瘤细胞中传递小干扰RNA（siRNA）的能力，从体内抗癌核酸药物传递的角度来看。 siRNA已被吸附到先前用阳离子聚合物涂层的NDs上。 利用嵌入的色心缺陷产生的NDs固有荧光，已证明NDs的细胞摄取。 这些涂层NDs的细胞毒性被证明是低的。 与内化效果一致，我们已经显示在含有血清的培养基中，由ND载体化的siRNA在mRNA和蛋白质水平上特异性抑制EWS/Fli-1基因表达。 显示英文摘要

摘要： We investigated the ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) in Ewing sarcoma cells, in the perspective of in vivo anti-cancer nucleic acid drug delivery. siRNA was adsorbed onto NDs previously coated with cationic polymer. Cell uptake of NDs has been demonstrated by taking advantage of NDs intrinsic fluorescence coming from embedded color center defects. Cell toxicity of these coated NDs was shown to be low. Consistent with the internalization efficacy, we have shown a specific inhibition of EWS/Fli-1 gene expression at the mRNA and protein level by the ND vectorized siRNA in a serum containing medium.