标题： 位点特异性重组：利用Cre-lox系统进行原位细胞条形码编码 显示英文标题

标题： Site-specific recombinatorics: in situ cellular barcoding with the Cre Lox system

摘要： 细胞条形码是一种重要的、最近开发的生物技术工具，它能够实现体内单个细胞后代的家族识别。 现有的大多数方法依赖于体外病毒转导细胞以引入条形码，然后进行过继转移进入动物体内，这种方法在某些系统中效果良好，但无法在天然环境中对细胞进行条形码标记，例如固体组织内的细胞。 为了克服这一限制，我们提出了一种基于Cre Lox系统的遗传条形码构建的新设计，该设计通过利用位点特异性重组过程中的固有序列距离约束，在细胞内原位诱导随机生成的稳定条形码。 利用这一之前未被使用的特性，我们确定了具有最大代码多样性的模块。 这证明其多样性比之前考虑的Cre Lox条形码方法所能达到的高几个数量级，并且由于其数量超过了小鼠中的淋巴细胞或造血祖细胞的数量，因此非常适合其预期的应用。 此外，它可以用现有的技术构建。 显示英文摘要

摘要： Cellular barcoding is a significant, recently developed, biotechnology tool that enables the familial identification of progeny of individual cells in vivo. Most existing approaches rely on ex vivo viral transduction of cells with barcodes, followed by adoptive transfer into an animal, which works well for some systems, but precludes barcoding cells in their native environment, such as those inside solid tissues. With a view to overcoming this limitation, we propose a new design for a genetic barcoding construct based on the Cre Lox system that induces randomly created stable barcodes in cells in situ by exploiting inherent sequence distance constraints during site-specific recombination. Leveraging this previously unused feature, we identify the cassette with maximal code diversity. This proves to be orders of magnitude higher than what is attainable with previously considered Cre Lox barcoding approaches and is well suited for its intended applications as it exceeds the number of lymphocytes or hematopoietic progenitor cells in mice. Moreover, it can be built using established technology.