标题： 无标记成像胆固醇和模型膜中脂质的分布 显示英文标题

标题： Label-free imaging of cholesterol and lipid distributions in model membranes

摘要： 近年来，脂质膜已成为研究细胞膜生物物理和生物化学的标准模型。 通过外源化学探针，通常使用荧光显微镜来研究生物膜中的脂质域。 然而，大多数荧光团的分配可调性有限，大多数会聚集在生物学上最不相关的域（即低密度液态域）。 因此，对形成膜的大部分未标记脂质的分子级图像仍然难以捉摸。 在此，我们展示了脂质单层中域形成的简单、无标签成像，并具有在所有域类型中解析脂质和胆固醇组成的化学选择性。 利用自发拉曼成像中的传统振动对比度，并结合化学计量学分析，可以检查包含饱和脂质、不饱和脂质和胆固醇的三元体系。 我们确认了荧光显微镜通常观察到的特征，并提供了在单膜水平上对胆固醇分布前所未有的分析。 显示英文摘要

摘要： Over recent decades, lipid membranes have become standard models for examining the biophysics and biochemistry of cell membranes. Interrogation of lipid domains within biomembranes is generally done with fluorescence microscopy via exogenous chemical probes. However, most fluorophores have limited partitioning tunability, with the majority segregating in the least biologically relevant domains (i.e., low-density liquid domains). Therefore, a molecular-level picture of the majority of non-labeled lipids forming the membrane is still elusive. Here, we present simple, label-free imaging of domain formation in lipid monolayers, with chemical selectivity in unraveling lipid and cholesterol composition in all domain types. Exploiting conventional vibrational contrast in spontaneous Raman imaging, combined with chemometrics analysis, allows for examination of ternary systems containing saturated lipids, unsaturated lipids, and cholesterol. We confirm features commonly observed by fluorescence microscopy, and provide an unprecedented analysis of cholesterol distribution at the single-membrane level.