标题： 定量相位成像中球形物体尺寸和折射率的准确评估 显示英文标题

标题： Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging

摘要： 测量定量相位成像（QPI）中悬浮细胞等球形物体的平均折射率（RI），需要从QPI数据中解耦RI和尺寸。 这通常通过几何方法确定物体的半径来实现，忽略光散射。 在此，我们提出了一种新的QPI拟合算法，该算法利用Mie理论和半解析校正的Rytov方法可靠地解耦RI。 我们通过计算机模拟评估了该算法的有效范围，并实验研究了各种物体（油滴和蛋白滴、微凝胶珠、细胞）和噪声条件。 此外，我们为细胞生物学的未来研究提供了重要的实用建议。 显示英文摘要

摘要： Measuring the average refractive index (RI) of spherical objects, such as suspended cells, in quantitative phase imaging (QPI) requires a decoupling of RI and size from the QPI data. This has been commonly achieved by determining the object's radius with geometrical approaches, neglecting light-scattering. Here, we present a novel QPI fitting algorithm that reliably uncouples the RI using Mie theory and a semi-analytical, corrected Rytov approach. We assess the range of validity of this algorithm in silico and experimentally investigate various objects (oil and protein droplets, microgel beads, cells) and noise conditions. In addition, we provide important practical cues for future studies in cell biology.