标题： ReCoDe：高通量时间分辨电子显微镜的数据缩减和压缩描述 显示英文标题

标题： ReCoDe: A Data Reduction and Compression Description for High Throughput Time-Resolved Electron Microscopy

摘要： 高速直接电子探测器显著提高了电子显微镜电影的时空分辨率。 然而，在长时间观测中保持空间和时间分辨率需要存储大量数据。 在这里，我们描述了一种高效且灵活的数据缩减和压缩方案（ReCoDe），通过保留单个电子事件来保持空间和时间分辨率。 在工作站上运行ReCoDe，我们展示了对从探测器流出的原始数据进行实时缩减和压缩，速度达到3 GB/s，持续数小时不间断的数据采集。 输出数据比原始数据小100倍，并通过10 GbE连接直接保存到网络附加存储驱动器上。 我们讨论了支持电子检测和计数的校准技术（例如，估计电子背散射率、误报率和数据可压缩性），以及由ReCoDe启用的新数据分析方法（例如，数据采集后的重新校准，以及对重叠损失的准确估计）。 显示英文摘要

摘要： Fast, direct electron detectors have significantly improved the spatio-temporal resolution of electron microscopy movies. Preserving both spatial and temporal resolution in extended observations, however, requires storing prohibitively large amounts of data. Here, we describe an efficient and flexible data reduction and compression scheme (ReCoDe) that retains both spatial and temporal resolution by preserving individual electron events. Running ReCoDe on a workstation we demonstrate on-the-fly reduction and compression of raw data streaming off a detector at 3 GB/s, for hours of uninterrupted data collection. The output was 100-fold smaller than the raw data and saved directly onto network-attached storage drives over a 10 GbE connection. We discuss calibration techniques that support electron detection and counting (e.g. estimate electron backscattering rates, false positive rates, and data compressibility), and novel data analysis methods enabled by ReCoDe (e.g. recalibration of data post acquisition, and accurate estimation of coincidence loss).