标题： 磷酸盐限制下集胞藻PCC 6803中Pho调节子的调控及光合装置的重建 显示英文标题

标题： Regulation of the Pho Regulon and Reconstruction of the Photosynthetic Apparatus under Phosphate Limitation in Synechocystis sp. PCC 6803

摘要： 在数千年中，蓝藻已经进化出适应动态环境的策略——这需要一个分子响应系统。 磷酸盐对于细胞完整性和代谢至关重要，然而无机磷酸盐通常是大多数水生环境中的限制性营养物。 本研究表征了两种周质磷酸盐结合蛋白，即 SphX 和 SphZ，它们对于 Synechocystis sp. PCC 6803 中 SphS-SphR 信号转导系统的功能响应是必不可少的，并将两者纳入一个模型中。 SphX 提供竞争性作用，以确保无机磷酸盐不会以比代谢所需的更快速度从环境中耗尽，因此会阻碍奢侈吸收；而 SphZ，由 sll0540 编码，是在磷酸盐限制条件下与磷酸盐特异性运输 2 系统和负调节因子 SphU 的蛋白质复合物相互作用时，SphS 响应所必需的辅助传感器。 还提出了 pho 调控子与光合装置之间的交叉调控，以及低温荧光发射光谱的参数化分析。 显示英文摘要

摘要： For millennia, cyanobacteria have evolved strategies for acclimatization to dynamic environments- this requires a molecular response system. Phosphate is essential for cellular integrity and metabolism, yet inorganic phosphate is often the limiting nutrient for most aquatic environments. This study characterizes two periplasmic phosphate-binding proteins, namely SphX and SphZ, which are essential for the functional response of the SphS-SphR signal transduction system in Synechocystis sp. PCC 6803 and incorporates both into a model. SphX offers a competitive role so as to ensure inorganic phosphate is not depleted from the environment at a faster rate than is necessary for metabolism and, therefore, hinders luxury uptake; whereas, SphZ, encoded by sll0540, is the auxiliary sensor necessary for the SphS response under phosphate limitation in tandem with a protein complex association with the phosphate-specific transport 2 system and the negative regulator, SphU. Cross-regulation between the pho regulon and the photosynthetic apparatus is presented as well as a parameterized analysis for low-temperature fluorescence emission spectroscopy.