标题： 宏菌孢子组成和生物分子图谱的定量评估通过拉曼光谱学 显示英文标题

标题： Quantitive evaluation of composition and biomolecular mapping of macrofungi spores by Raman spectroscopy

摘要： 拉曼光谱被广泛应用于检测有机物中的不同化学化合物，并在单个细胞水平上创建无标记的高分辨率图谱。这种技术的主要优势是能够在不进行特殊预处理的情况下在体内研究物体。然而，它很少用于确定不同物质之间的比例或对所得光谱进行深入的定量分析。在我们的研究中，我们推导出比率方程，这些方程允许估算三酰甘油、蛋白质、糖类、多糖和DNA的质量浓度。我们表明，可以从脂质的光谱中确定平均不饱和度和链长，以及从蛋白质的光谱中确定苯丙氨酸、酪氨酸和色氨酸的浓度。我们将推导出的方程应用于超过70种大型真菌的真菌孢子的拉曼光谱。我们提供了对孢子中所含脂质、蛋白质和多糖的详细表征。我们认为，我们的研究不仅为真菌学领域提供了新的基础性知识，而且可以成为任何有机材料光谱定量的基础，我们的方法可以应用于例如食品诊断。 显示英文摘要

摘要： Raman spectroscopy is widely applied to detect different chemical compounds in organic matter and create label-free high-resolution maps on the level of separate cells. The main advantage of this technique is the possibility to study objects in vivo without special pretreatment. However, it is rarely used to determine ratios between different substances or to perform deep quantitative analysis of the obtained spectra. In our study, we derive ratiometric equations that allow estimating the mass concentrations of triacylglycerols, proteins, sugars, polysaccharides, and DNA. We show that it is possible to determine average unsaturation and chain length from the spectra of lipids and concentrations of phenylalanine, tyrosine, and tryptophan from the spectra of proteins. We apply the derived equations to the Raman spectra of fungal spores of over 70 different species of macrofungi. We provide detailed characterization of lipids, proteins, and polysaccharides, which are contained in the spores. We believe that our study not only provides new fundamental knowledge in the field of mycology but also can become a basis for spectral quantification of any organic material, and our approach can be applied, for instance, to food diagnostics.