标题： 用光等离子体耳语峡谷模式生物传感器的顺序模式监测单个DNA接合位点活性 显示英文标题

标题： Monitoring Single DNA Docking Site Activity With Sequential Modes of an Optoplasmonic Whispering-Gallery Mode Biosensor

摘要： 近年来，单分子技术取得了快速进展，这得益于它们在研究超出衍射极限的分子时所表现出的无与伦比的精度。 在这些技术中，光等离子体驻波腔模式传感在无标记的单分子表征方面展现出了巨大的潜力。 它结合了局域表面等离子体共振（LSPR）和驻波腔模式（WGM）传感的原理，即使在单个离子的水平上也提供了卓越的传感能力。 然而，当前的光等离子体WGM传感是在多通道中运行的，使得专注于分析物分子的单个结合位点变得具有挑战性。 在本文中，我们使用连续极化WGM模式之间的共振偏移比，对DNA分析物分子与光等离子体WGM传感器上的锚定链杂交的不同结合位点进行表征。 我们识别出特定的锚定位点，这些位点经历短暂的相互作用，并最终与互补的分析物链永久杂交。 显示英文摘要

摘要： In recent years, there has been rapid advancement in single-molecule techniques, driven by their unparalleled precision in studying molecules whose sizes are beyond the diffraction limit. Among these techniques, optoplasmonic whispering gallery mode sensing has demonstrated great potential in label-free single-molecule characterization. It combines the principles of localized surface plasmon resonance (LSPR) and whispering gallery mode (WGM) sensing, offering exceptional sensing capabilities, even at the level of single ions. However, current optoplasmonic WGM sensing operates in a multiplexed channel, making it challenging to focus on individual binding sites of analyte molecules. In this article, we characterize different binding sites of DNA analyte molecules hybridizing to docking strands on the optoplasmonic WGM sensor, using the ratio of the resonance shift between sequential polar WGM modes. We identify specific docking sites that undergo transient interactions and eventually hybridize with the complementary analyte strands permanently.