标题： 荧光寿命成像显微镜分析孤立的黑素体 显示英文标题

标题： Fluorescence Lifetime Imaging Microscopy Analysis of Isolated Melanosomes

摘要： 黑素体是存在于动物界各种组织中的细胞器。 它们含有多种生物分子，但主要成分是色素黑色素，许多归因于黑素体的功能，如光保护，都是由其所含黑色素的化学特性和结构独特地实现的。 在本报告中，我们首次使用荧光寿命成像显微镜（FLIM）来研究黑素体中色素的荧光特性，并评估长时间激光照射后的时间演变。 我们发现荧光发射中存在一个相对较短寿命的组分，并揭示了在照射后寿命发生显著变化，表明黑色素在几分钟的时间尺度上发生了结构上的光诱导变化，观察持续时间最长可达一小时。 显示英文摘要

摘要： Melanosomes are organelles found in a wide variety of tissues throughout the animal kingdom. They contain a variety of biological molecules, but the dominant constituent is the pigment melanin, and many functions ascribed to melanosomes, such as photoprotection, are uniquely enabled by the chemical properties and structures of the melanins they contain. In this report, we used, for the first time, Fluorescence Lifetime Imaging Microscopy (FLIM) to examine fluorescent properties of pigments in melanosomes and evaluate their time evolution upon extended laser irradiation. We discovered a relatively short-lived component in fluorescence emission and revealed significant changes in lifetimes upon irradiation indicating structural photoinduced changes to melanin occurring on a time scale of minutes, with observations extending up to one hour.