标题： 使用微毛细管阵列的表达构建体高通量筛选 显示英文标题

标题： High Throughput Screening of Expression Constructs using Microcapillary Arrays

摘要： 基因表达是一个涉及众多相互关联变量的复杂现象，研究这些变量以控制表达在生物工程和生物制造中是至关重要的。 尽管存在用于实现覆盖大设计空间的质粒文库的克隆技术，但能够以相似规模进行细胞培养筛选的多重技术仍然缺乏。 我们引入了一种基于微毛细管阵列的平台，旨在通过荧光报告基因对微型细胞培养物进行高通量、多重筛选。 显示英文摘要

摘要： Gene expression is a complex phenomenon involving numerous interlinked variables, and studying these variables to control expression is essential in bioengineering and biomanufacturing. While cloning techniques for achieving plasmid libraries that cover large design spaces exist, multiplex techniques offering cell culture screening at similar scales are still lacking. We introduced a microcapillary array-based platform aimed at high-throughput, multiplex screening of miniature cell cultures through fluorescent reporters.