标题： 蛋白质在自由溶液中的单图像扩散系数测量 显示英文标题

标题： Single-image diffusion coefficient measurements of proteins in free solution

摘要： 扩散系数测量对于许多生物和材料研究非常重要，例如粒子动力学、反应动力学和尺寸测定。在现有的测量方法中，单粒子追踪（SPT）提供了同时提供分子位置和扩散信息的独特能力，同时仅需飞摩尔的样品。然而，对于单色标记样品，SPT的时间分辨率限制为秒级。通过直接成像三维（3D）扩散的荧光蛋白并研究其强度分布的宽度，我们使用亚毫秒曝光时间的单蛋白图像确定蛋白质的扩散系数。这种方法简单，将扩散系数测量的时间分辨率提高到亚毫秒级别，并可方便地应用于SPT研究和应用中需要扩散系数测量的范围，例如反应动力学和粒子尺寸测定。 显示英文摘要

摘要： Diffusion coefficient measurements are important for many biological and material investigations, such as particle dynamics, kinetics, and size determinations. Amongst current measurement methods, single particle tracking (SPT) offers the unique capability of providing location and diffusion information of a molecule simultaneously while using only femptomoles of sample. However, the temporal resolution of SPT is limited to seconds for single-color labeled samples. By directly imaging three dimensional (3D) diffusing fluorescent proteins and studying the widths of their intensity profiles, we determine the proteins' diffusion coefficients using single protein images of sub-millisecond exposure times. This simple method improves the temporal resolution of diffusion coefficient measurements to sub-millisecond, and can be readily applied to a range of particle sizes in SPT investigations and applications where diffusion coefficient measurements are needed, such as reaction kinetics and particle size determinations.